Ovine oocytes to assess sperm functionality

Fertilizing capacity of spermatozoa deposited into the fallopian tubes. After 5 min, blastocysts were transferred to cryobuffer medium containing 0. Conclusions In summary, results obtained with our newly produced rHamOVGP1 showed that addition of rHamOVGP1 in the capacitation medium further enhanced tyrosine phosphorylation of a sub-set of sperm proteins and significantly increased acrosome reaction. The present investigation was undertaken in order to evaluate two ram sperm capacitation media by zona-free hamster penetration test, and to determine the acrosome reaction rate from capacitated spermatozoa, providing a useful capacitation technique for ovine in vitro fertilization. This confirmed previous reports in cattle
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Acta Scientiae Veterinariae

Capacitation status of fresh and frozen-thawed buffalo spermatozoa in relation to cholesterol level, membrane fluidity and intracellular calcium. Services Email this article to a colleague Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Request permission. Purification of secreted rHamOVGP1 from serum-free cell culture medium by affinity chromatography Purification of rHamOVGP1 was performed using lectin-affinity chromatography as previously described [ 36 ]. Semen collection and freezing Ejaculates were collected by artificial vagina from two Merino rams Ram 1, Ram 2. Under a Creative Commons license. Abstract Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Reproduction, Nutrition, Development 36 21 —
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Results were consistent for all analyses. Another third was flow cytometrically sorted and either assessed after sorting FS or re-frozen and thawed FSF. In previous studies that used BCB for selecting oocytes, an in vitro culture channel was generally highlighted to verify its effectiveness. The present results demonstrate clearly that the effect of rHamOVGP1 on protein tyrosine phosphorylation PYP during capacitation is dose- and time-dependent. Ovine in vitro embryo production and in vivo surgical recovery were performed as described Effect of Sperm Composition. Fresh mouse sperm was used.
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Journal of Animal Science 78 Suppl 1 An Oocyte Demethylation Activity? Embryo vitrification and re-warming Day 6 and 7 blastocysts were cryopreserved using the open-pulled straw vitrification method Vajta et al. Alternatively, species differences in the distribution of methylated DNA may determine whether a major male pronuclear demethylation event is required. On the other hand, oocytes Under a Creative Commons license.
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